What are the different techniques of pipetting?
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There are mainly two techniques of pipetting:
- Forward pipetting technique: Forward pipetting is a technique for dispensing a precise amount of liquid by means of an air displacement pipette. The technique is primarily recommended for aqueous solutions, such as buffers, or dilute acids or alkalis.
- Reverse pipetting technique: Reverse pipetting is a technique for dispensing a precise amount of liquid by means of an air displacement pipette. This technique is mainly used for solutions with high viscosity or a tendency to foam as it minimizes the risk of splashing, foam, or bubble formation. Reverse piping is more accurate in dispensing small amounts of liquids containing proteins and biological solutions.
